TY  - JOUR
A1  - Zintel, Sandra
A1  - Bernhardt, Dominik
A1  - Rogowska-Wrzesinska, Adelina
A1  - Osiewacz, Heinz D.
T1  - PaCATB : a secreted catalase protecting Podospora anserina against exogenous oxidative stress
T2  - Aging
N2  - A differential mass spectrometry analysis of secreted proteins from juvenile and senescentPodospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.
KW  - Podospora anserina
KW  - secreted proteins
KW  - oxidative stress
KW  - aging and catalases
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/25940
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-259401
SN  - 1945-4589
VL  - 3
IS  - 8
SP  - 768
EP  - 781
PB  - Impact Journals, LLC
CY  - [S.l.]
ER  -