TY  - JOUR
A1  - Milovic, Vladan
A1  - Turchanowa, Lyudmila
A1  - Stein, Jürgen
A1  - Caspary, Wolfgang F.
T1  - Transepithelial transport of putrescine across monolayers of the human intestinal epithelial cell line, Caco-2
T2  - World journal of gastroenterology
N2  - Aim: To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.
Methods: The transepithelial transport and the cellular accumulation of putrescine was measured using Caco-2 cell monolayers grown on permeable filters.
Results: Transepithelial transport of putrescine in physiological concentrations ( > 0.5 mM) from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical to basolateral direction.EGF enhanced putrescine accumulation in Caco-2 cells by four fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of S adenosylmethionine decarboxylase. However, EGF did not have any significant influence on putrescine flux across the Caco- 2 cell monolayers. Excretion of putrescine from Caco-2 cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber, contributed hundreds of micromoles polyamines to the basolateral chamber.
Conclusion: Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.
KW  - putrescine/metabolism
KW  - Caco 2 cells/metabolism
KW  - polyamines/metabolism
KW  - biological transport
Y1  - 2001
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45620
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-456206
SN  - 2219-2840
SN  - 1007-9327
N1  - This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
VL  - 7
IS  - 2
SP  - 193
EP  - 197
PB  - WJG Press
CY  - Beijing
ER  -