TY  - JOUR
A1  - Söhling, Nicolas
A1  - Neijhoft, Jonas
A1  - Nienhaus, Vinzenz
A1  - Acker, Valentin
A1  - Harbig, Jana
A1  - Menz, Fabian
A1  - Ochs, Joachim
A1  - Verboket, René
A1  - Ritz, Ulrike
A1  - Blaeser, Andreas
A1  - Dörsam, Edgar
A1  - Frank, Johannes
A1  - Marzi, Ingo
A1  - Henrich, Dirk
T1  - 3d-printing of hierarchically designed and osteoconductive bone tissue engineering scaffolds
T2  - Materials
N2  - In Bone Tissue Engineering (BTE), autologous bone-regenerative cells are combined with a scaffold for large bone defect treatment (LBDT). Microporous, polylactic acid (PLA) scaffolds showed good healing results in small animals. However, transfer to large animal models is not easily achieved simply by upscaling the design. Increasing diffusion distances have a negative impact on cell survival and nutrition supply, leading to cell death and ultimately implant failure. Here, a novel scaffold architecture was designed to meet all requirements for an advanced bone substitute. Biofunctional, porous subunits in a load-bearing, compression-resistant frame structure characterize this approach. An open, macro- and microporous internal architecture (100 µm–2 mm pores) optimizes conditions for oxygen and nutrient supply to the implant’s inner areas by diffusion. A prototype was 3D-printed applying Fused Filament Fabrication using PLA. After incubation with Saos-2 (Sarcoma osteogenic) cells for 14 days, cell morphology, cell distribution, cell survival (fluorescence microscopy and LDH-based cytotoxicity assay), metabolic activity (MTT test), and osteogenic gene expression were determined. The adherent cells showed colonization properties, proliferation potential, and osteogenic differentiation. The innovative design, with its porous structure, is a promising matrix for cell settlement and proliferation. The modular design allows easy upscaling and offers a solution for LBDT.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54344
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-543443
SN  - 1996-1944
VL  - 13
IS  - 1836
SP  - 1
EP  - 18
PB  - MDPI
CY  - Basel
ER  -