TY  - JOUR
A1  - Wilhelm, Alexander
A1  - Toptan, Tuna
A1  - Pallas, Christiane
A1  - Wolf, Timo
A1  - Götsch, Udo
A1  - Gottschalk, René
A1  - Vehreschild, Maria J. G. T.
A1  - Ciesek, Sandra
A1  - Widera, Marek
T1  - Antibody-mediated neutralization of authentic SARS-CoV-2 B.1.617 variants harboring L452R and T478K/E484Q
T2  - Viruses
N2  - The capacity of convalescent and vaccine-elicited sera and monoclonal antibodies (mAb) to neutralize SARS-CoV-2 variants is currently of high relevance to assess the protection against infections. We performed a cell culture-based neutralization assay focusing on authentic SARS-CoV-2 variants B.1.617.1 (Kappa), B.1.617.2 (Delta), B.1.427/B.1.429 (Epsilon), all harboring the spike substitution L452R. We found that authentic SARS-CoV-2 variants harboring L452R had reduced susceptibility to convalescent and vaccine-elicited sera and mAbs. Compared to B.1, Kappa and Delta showed a reduced neutralization by convalescent sera by a factor of 8.00 and 5.33, respectively, which constitutes a 2-fold greater reduction when compared to Epsilon. BNT2b2 and mRNA1273 vaccine-elicited sera were less effective against Kappa, Delta, and Epsilon compared to B.1. No difference was observed between Kappa and Delta towards vaccine-elicited sera, whereas convalescent sera were 1.51-fold less effective against Delta, respectively. Both B.1.617 variants Kappa (+E484Q) and Delta (+T478K) were less susceptible to either casirivimab or imdevimab. In conclusion, in contrast to the parallel circulating Kappa variant, the neutralization efficiency of convalescent and vaccine-elicited sera against Delta was moderately reduced. Delta was resistant to imdevimab, which, however, might be circumvented by combination therapy with casirivimab together.
KW  - SARS-CoV-2
KW  - delta
KW  - Kappa
KW  - Epsilon
KW  - B.1.617.1
KW  - B.1.617.2
KW  - corona virus
KW  - monoclonal antibodies
KW  - vaccination
KW  - BNT2b2
KW  - mRNA1273
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63446
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-634460
SN  - 1999-4915
N1  - This study has been performed with the support of the Goethe-Corona-Fund of the Goethe University Frankfurt (MW) and the Federal Ministry of Education and Research (COVIDready; grant 02WRS1621C (MW).
VL  - 13
IS  - 9, art. 1693
SP  - 1
EP  - 8
PB  - MDPI
CY  - Basel
ER  -