TY  - JOUR
A1  - Scheckhuber, Christian Quintus
A1  - Grief, Jürgen
A1  - Boilan, Emmanuelle
A1  - Luce, Karin
A1  - Debacq-Chainiaux, Florence
A1  - Rittmeyer, Claudia
A1  - Gredilla, Ricardo
A1  - Kolbesen, Bernd O.
A1  - Toussaint, Olivier
A1  - Osiewacz, Heinz D.
T1  - Age-related cellular copper dynamics in the fungal ageing model Podospora anserina and in ageing human fibroblasts
T2  - PLoS One
N2  - In previous investigations an impact of cellular copper homeostasis on ageing of the ascomycete Podospora anserina has been demonstrated. Here we provide new data indicating that mitochondria play a major role in this process. Determination of copper in the cytosolic fraction using total reflection X-ray fluorescence spectroscopy analysis and eGfp reporter gene studies indicate an age-related increase of cytosolic copper levels. We show that components of the mitochondrial matrix (i.e. eGFP targeted to mitochondria) become released from the organelle during ageing. Decreasing the accessibility of mitochondrial copper in P. anserina via targeting a copper metallothionein to the mitochondrial matrix was found to result in a switch from a copper-dependent cytochrome-c oxidase to a copper-independent alternative oxidase type of respiration and results in lifespan extension. In addition, we demonstrate that increased copper concentrations in the culture medium lead to the appearance of senescence biomarkers in human diploid fibroblasts (HDFs). Significantly, expression of copper-regulated genes is induced during in vitro ageing in medium devoid of excess copper suggesting that cytosolic copper levels also increase during senescence of HDFs. These data suggest that the identified molecular pathway of age-dependent copper dynamics may not be restricted to P. anserina but may be conserved from lower eukaryotes to humans.
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/20117
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-83895
SN  - 1932-6203
N1  - Copyright: © 2009 Scheckhuber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 4
IS  - (3): e4919
ER  -