TY  - JOUR
A1  - Richter, Christian
A1  - Kovacs, Helena
A1  - Buck, Janina
A1  - Wacker, Anna
A1  - Fürtig, Boris
A1  - Bermel, Wolfgang
A1  - Schwalbe, Harald
T1  - 13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides
T2  - Journal of biomolecular NMR
N2  - We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose 13C-spins, (2) the (H)CPC-experiment that correlates each phosphorus with the C40 nuclei of adjacent nucleotides via J(C,P) couplings and (3) the (H)CPC-CCH-TOCSY-experiment that correlates the phosphorus nuclei with the respective C10,H10 ribose signals. The experiments were applied to two RNA hairpin structures. The current set of 13C-direct detected experiments allows direct and unambiguous assignment of the majority of the hetero nuclei and the identification of the individual ribose moieties following their sequential assignment. Thus, 13C-direct detected NMR methods constitute useful complements to the conventional 1H-detected approach for the resonance assignment of oligonucleotides that is often hindered by the limited chemical shift dispersion. The developed methods can also be applied to large deuterated RNAs. Keywords: NMR spectroscopy , Direct carbon , detection , RNA
KW  - NMR spectroscopy
KW  - Direct carbon
KW  - detection
KW  - RNA
Y1  - 2010
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/20243
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-85015
SN  - 0925-2738
SN  - 1573-5001
N1  - Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
VL  - 47
IS  - 4
SP  - 259
EP  - 269
PB  - Springer
CY  - Dordrecht
ER  -