TY  - JOUR
A1  - Herzog, Christine
A1  - Schmitz, Martina
A1  - Levkau, Bodo
A1  - Herrgott, Ilka
A1  - Mersmann, Jan
A1  - Larmann, Jan
A1  - Johanning, Kai
A1  - Winterhalter, Michael
A1  - Chun, Jerold
A1  - Müller, Frank Ulrich
A1  - Echtermeyer, Frank
A1  - Hildebrand, Reinhard
A1  - Theilmeier, Gregor
T1  - Intravenous sphingosylphosphorylcholine protects ischemic and postischemic myocardial tissue in a mouse model of myocardial ischemia/reperfusion injury
T2  - Mediators of inflammation
N2  - HDL, through sphingosine-1-phosphate (S1P), exerts direct cardioprotective effects on ischemic myocardium. It remains unclear whether other HDL-associated sphingophospholipids have similar effects. We therefore examined if HDL-associated sphingosylphosphorylcholine (SPC) reduces infarct size in a mouse model of transient myocardial ischemia/reperfusion. Intravenously administered SPC dose-dependently reduced infarct size after 30 minutes of myocardial ischemia and 24 hours reperfusion compared to controls. Infarct size was also reduced by postischemic, therapeutical administration of SPC. Immunohistochemistry revealed reduced polymorphonuclear neutrophil recruitment to the infarcted area after SPC treatment, and apoptosis was attenuated as measured by TUNEL. In vitro, SPC inhibited leukocyte adhesion to TNFα-activated endothelial cells and protected rat neonatal cardiomyocytes from apoptosis. S1P3 was identified as the lysophospholipid receptor mediating the cardioprotection by SPC, since its effect was completely absent in S1P3-deficient mice. We conclude that HDL-associated SPC directly protects against myocardial reperfusion injury in vivo via the S1P3 receptor.
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/20673
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-89145
SN  - 1466-1861
SN  - 0962-9351
N1  - Copyright © 2010 Christine Herzog et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2010
IS  - Art. 425191
SP  - 1
EP  - 7
PB  - Hindawi Publishing Corp.
CY  - Sylvania, Ohio
ER  -