TY - JOUR A1 - Nijkamp, Jurgen F. A1 - Broek, Marcel van den A1 - Datema, Erwin A1 - Kok, Stefan de A1 - Bosman, Lizanne A1 - Luttik, M. Louise A1 - Daran-Lapujade, Pascale A1 - Vongsangnak, Wanwipa A1 - Nielsen, Jens A1 - Heijne, Wilbert H. M. A1 - Klaassen, Paul A1 - Paddon, Chris J. A1 - Platt, Darren A1 - Kötter, Peter A1 - Ham, Roeland C. van A1 - Reinders, Marcel J. T. A1 - Pronk, Jack T. A1 - Ridder, Dick de A1 - Daran, Jean-Marc T1 - De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology T2 - Microbial cell factories N2 - Saccharomyces cerevisiae CEN.PK 113-7D is widely used for metabolic engineering and systems biology research in industry and academia. We sequenced, assembled, annotated and analyzed its genome. Single-nucleotide variations (SNV), insertions/deletions (indels) and differences in genome organization compared to the reference strain S. cerevisiae S288C were analyzed. In addition to a few large deletions and duplications, nearly 3000 indels were identified in the CEN.PK113-7D genome relative to S288C. These differences were overrepresented in genes whose functions are related to transcriptional regulation and chromatin remodelling. Some of these variations were caused by unstable tandem repeats, suggesting an innate evolvability of the corresponding genes. Besides a previously characterized mutation in adenylate cyclase, the CEN.PK113-7D genome sequence revealed a significant enrichment of non-synonymous mutations in genes encoding for components of the cAMP signalling pathway. Some phenotypic characteristics of the CEN.PK113-7D strains were explained by the presence of additional specific metabolic genes relative to S288C. In particular, the presence of the BIO1 and BIO6 genes correlated with a biotin prototrophy of CEN.PK113-7D. Furthermore, the copy number, chromosomal location and sequences of the MAL loci were resolved. The assembled sequence reveals that CEN.PK113-7D has a mosaic genome that combines characteristics of laboratory strains and wild-industrial strains. Y1 - 2012 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/23509 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-235097 SN - 1475-2859 N1 - © 2012 Nijkamp et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. VL - 11 IS - Art. 36 SP - 1 EP - 16 PB - BioMed Central CY - London ER -