TY  - JOUR
A1  - Burmeister, Thomas
A1  - Molkentin, Mara
A1  - Meyer, Claus
A1  - Lachmann, Nils
A1  - Schwartz, Stefan
A1  - Friedrichs, Birte
A1  - Beyer, Jörg
A1  - Blau, Igor Wolfgang
A1  - Lohm, Gunnar
A1  - Tietze-Bürger, Carola
A1  - Marschalek, Rolf
A1  - Uharek, Lutz
T1  - Molecular monitoring of minimal residual disease in two patients with MLL-rearranged acute myeloid leukemia and haploidentical transplantation after relapse
T2  - Experimental hematology & oncology
N2  - This report describes the clinical courses of two acute myeloid leukemia patients. Both had MLL translocations, the first a t(10;11)(p11.2;q23) with MLL-AF10 and the second a t(11;19)(q23;p13.1) with MLL-ELL fusion. They achieved a clinical remission under conventional chemotherapy but relapsed shortly after end of therapy. Both had a history of invasive mycoses (one had possible pulmonary mycosis, one systemic candidiasis). Because no HLA-identical donor was available, a haploidentical transplantation was performed in both cases. Using a specially designed PCR method for the assessment of minimal residual disease (MRD), based on the quantitative detection of the individual chromosomal breakpoint in the MLL gene, all patients achieved complete and persistent molecular remission after transplantation. The immune reconstitution after transplantation is described in terms of total CD3+/CD4+, CD3+/CD8+, CD19+, and CD16+/CD56+ cell numbers over time. The KIR and HLA genotypes of donors and recipients are reported and the possibility of a KIR-mediated alloreactivity is discussed. This report illustrates that haploidentical transplantation may offer a chance of cure without chronic graft-versus-host disease in situations where no suitable HLA-identical donor is available even in a high-risk setting and shows the value of MRD monitoring in the pre- and posttransplant setting.
KW  - Minimal residual disease
KW  - Haploidentical stem cell transplantation
KW  - MLL
KW  - KIR
KW  - Acute myeloid leukemia
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/23742
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-237427
SN  - 2162-3619
VL  - 1
IS  - 6
PB  - BioMed Central
CY  - London
ER  -