TY - JOUR A1 - Poehlein, Anja A1 - Schmidt, Silke A1 - Kaster, Anne-Kristin A1 - Goenrich, Meike A1 - Vollmers, John A1 - Thürmer, Andrea A1 - Bertsch, Johannes A1 - Schuchmann, Kai A1 - Voigt, Birgit A1 - Hecker, Michael A1 - Daniel, Rolf A1 - Thauer, Rudolf K. A1 - Gottschalk, Gerhard A1 - Müller, Volker T1 - An ancient pathway combining carbon dioxide fixation with the generation and utilization of a sodium ion gradient for ATP synthesis T2 - PLoS One N2 - Synthesis of acetate from carbon dioxide and molecular hydrogen is considered to be the first carbon assimilation pathway on earth. It combines carbon dioxide fixation into acetyl-CoA with the production of ATP via an energized cell membrane. How the pathway is coupled with the net synthesis of ATP has been an enigma. The anaerobic, acetogenic bacterium Acetobacterium woodii uses an ancient version of this pathway without cytochromes and quinones. It generates a sodium ion potential across the cell membrane by the sodium-motive ferredoxin:NAD oxidoreductase (Rnf). The genome sequence of A. woodii solves the enigma: it uncovers Rnf as the only ion-motive enzyme coupled to the pathway and unravels a metabolism designed to produce reduced ferredoxin and overcome energetic barriers by virtue of electron-bifurcating, soluble enzymes. Y1 - 2012 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/24643 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-246436 SN - 1932-6203 VL - 7 IS - 3: e32946 PB - PLoS CY - Lawrence, Kan. ER -