TY  - JOUR
A1  - Althoff, Thorsten
A1  - Mills, Deryck J.
A1  - Popot, Jean-Luc
A1  - Kühlbrandt, Werner
T1  - Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1
T2  - The EMBO journal
N2  - The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 1.7-MDa amphipol-solubilized supercomplex I1III2IV1 from bovine heart obtained by single-particle electron cryo-microscopy reveals an amphipol belt replacing the membrane lipid bilayer. A precise fit of the X-ray structures of complex I, the complex III dimer, and monomeric complex IV indicates distances of 13 nm between the ubiquinol-binding sites of complexes I and III, and of 10–11 nm between the cytochrome c binding sites of complexes III and IV. The arrangement of respiratory chain complexes suggests two possible pathways for efficient electron transfer through the supercomplex, of which the shorter branch through the complex III monomer proximal to complex I may be preferred.
KW  - amphipols
KW  - electron cryo-microscopy
KW  - membrane protein complex
KW  - respiratory chain
KW  - single-particle averaging
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/25929
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-259298
SN  - 0261-4189
SN  - 1460-2075
VL  - 30
SP  - 4652
EP  - 4664
PB  - Nature Publ. Group
CY  - London
ER  -