TY  - JOUR
A1  - Endesfelder, Ulrike
A1  - Malkusch, Sebastian
A1  - Flottmann, Benjamin
A1  - Mondry, Justine
A1  - Liguzinski, Piotr
A1  - Verveer, Peter J.
A1  - Heilemann, Mike
T1  - Chemically induced photoswitching of fluorescent probes : a general concept for super-resolution microscopy
T2  - Molecules
N2  - We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.
KW  - photoswitchable organic fluorophores
KW  - fluorescent proteins
KW  - super-resolution
KW  - PALM
KW  - dSTORM
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/34810
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-348104
SN  - 1420-3049
N1  - © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
VL  - 16
IS  - 4
SP  - 3106
EP  - 3118
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -