TY  - JOUR
A1  - Kim-Wanner, Soo-Zin
A1  - Bug, Gesine
A1  - Steinmann, Juliane
A1  - Ajib, Salem
A1  - Sorg, Nadine
A1  - Poppe, Carolin
A1  - Bunos, Milica
A1  - Wingenfeld, Eva
A1  - Hümmer, Christiane
A1  - Luxembourg, Beate
A1  - Seifried, Erhard
A1  - Bönig, Halvard-Björn
T1  - Erythrocyte depletion from bone marrow : performance evaluation after 50 clinical-scale depletions with Spectra Optia BMC
T2  - Journal of translational medicine
N2  - Background: Red blood cell (RBC) depletion is a standard graft manipulation technique for ABO-incompatible bone marrow (BM) transplants. The BM processing module for Spectra Optia, “BMC”, was previously introduced. We here report the largest series to date of routine quality data after performing 50 clinical-scale RBC-depletions.
Methods: Fifty successive RBC-depletions from autologous (n = 5) and allogeneic (n = 45) BM transplants were performed with the Spectra Optia BMC apheresis suite. Product quality was assessed before and after processing for volume, RBC and leukocyte content; RBC-depletion and stem cell (CD34+ cells) recovery was calculated there from. Clinical engraftment data were collected from 26/45 allogeneic recipients.
Results: Median RBC removal was 98.2% (range 90.8–99.1%), median CD34+ cell recovery was 93.6%, minimum recovery being 72%, total product volume was reduced to 7.5% (range 4.7–23.0%). Products engrafted with expected probability and kinetics. Performance indicators were stable over time.
Discussion: Spectra Optia BMC is a robust and efficient technology for RBC-depletion and volume reduction of BM, providing near-complete RBC removal and excellent CD34+ cell recovery.
KW  - Cell processing
KW  - RBC-depletion
KW  - Apheresis
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43959
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-439594
SN  - 1479-5876
N1  - © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
VL  - 15
IS  - 1, Art. 174
SP  - 1
EP  - 7
PB  - BioMed Central
CY  - London
ER  -