TY  - JOUR
A1  - Pollmann, Hendrik
A1  - Breitenbach, Jürgen
A1  - Wolff, Hendrik
A1  - Bode, Helge Björn
A1  - Sandmann, Gerhard
T1  - Combinatorial biosynthesis of novel multi-hydroxy carotenoids in the red yeast xanthophyllomyces dendrorhous
T2  - Journal of Fungi
N2  - The red yeast Xanthophyllomyces dendrorhous is an established platform for the synthesis of carotenoids. It was used for the generation of novel multi oxygenated carotenoid structures. This was achieved by a combinatorial approach starting with the selection of a β-carotene accumulating mutant, stepwise pathway engineering by integration of three microbial genes into the genome and finally the chemical reduction of the resulting 4,4’-diketo-nostoxanthin (2,3,2’,3’-tetrahydroxy-4,4’-diketo-β-carotene) and 4-keto-nostoxanthin (2,3,2’,3’-tetrahydroxy-4-monoketo-β-carotene). Both keto carotenoids and the resulting 4,4’-dihydroxy-nostoxanthin (2,3,4,2’,3’,4’-hexahydroxy-β-carotene) and 4-hydroxy-nostoxanthin (2,3,4,2’3’-pentahydroxy-β-carotene) were separated by high-performance liquid chromatography (HPLC) and analyzed by mass spectrometry. Their molecular masses and fragmentation patterns allowed the unequivocal identification of all four carotenoids.
KW  - carotenoid biosynthesis
KW  - 4,4’-dihydroxy-nostoxanthin
KW  - 4,4’-diketo-nostoxanthin
KW  - genetic engineering
KW  - HPLC separation
KW  - MS-MS spectra
KW  - Xanthophyllomyces dendrorhous
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44232
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-442321
SN  - 2309-608X
N1  - © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 3
IS  - 1, Art. 9
SP  - 1
EP  - 10
PB  - MDPI
CY  - Basel
ER  -