TY  - JOUR
A1  - Suzuki, Nobuhiro
A1  - Rohaim, Ahmed
A1  - Kato, Ryuichi
A1  - Đikić, Ivan
A1  - Wakatsuki, Soichi
A1  - Kawasaki, Masato
T1  - A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1
T2  - The FEBS journal
N2  - The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. Here, we report the crystal structure of the UBZ domain of Y-family DNA polymerase (pol) η and the crystal structure of the complex between the UBZ domain of Werner helicase-interacting protein 1 (WRNIP1) and ubiquitin, crystallized using the GFP fusion technique. In contrast to the pol η UBZ, which has been proposed to bind ubiquitin via its C-terminal α-helix, ubiquitin binds to a novel surface of WRNIP1 UBZ composed of the first β-strand and the C-terminal α-helix. In addition, we report the structure of the tandem UBZ domains of Tax1-binding protein 1 (TAX1BP1) and show that the second UBZ of TAX1BP1 binds ubiquitin, presumably in a manner similar to that of WRNIP1 UBZ. We propose that UBZ domains can be divided into at least two different types in terms of the ubiquitin-binding surfaces: the pol η type and the WRNIP1 type.
KW  - green fluorescent protein
KW  - pol g
KW  - TAX1BP1
KW  - ubiquitin-binding zinc finger
KW  - WRNIP1
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44303
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-443039
SN  - 1742-4658
SN  - 1742-464X
N1  - © 2016 Federation of European Biochemical Societies
VL  - 283
IS  - 11
SP  - 2004
EP  - 2017
PB  - Wiley-Blackwell
CY  - Oxford [u. a.]
ER  -