TY  - JOUR
A1  - Störmann, Philipp
A1  - Auner, Birgit
A1  - Schimunek, Lukas
A1  - Serve, Rafael Felix
A1  - Horst, Klemens
A1  - Simon, Tim-Philipp
A1  - Pfeifer, Roman
A1  - Köhler, Kernt
A1  - Hildebrand, Frank
A1  - Wutzler, Sebastian
A1  - Pape, Hans-Christoph
A1  - Marzi, Ingo
A1  - Relja, Borna
T1  - Leukotriene B4 indicates lung injury and on-going inflammatory changes after severe trauma in a porcine long-term model
T2  - Prostaglandins, leukotrienes and essential fatty acids
N2  - Background: Recognizing patients at risk for pulmonary complications (PC) is of high clinical relevance. Migration of polymorphonuclear leukocytes (PMN) to inflammatory sites plays an important role in PC, and is tightly regulated by specific chemokines including interleukin (IL)−8 and other mediators such as leukotriene (LT)B4. Previously, we have reported that LTB4 indicated early patients at risk for PC after trauma. Here, the relevance of LTB4 to indicating lung integrity in a newly established long-term porcine severe trauma model (polytrauma, PT) was explored.
Methods: mTwelve pigs (3 months old, 30 ± 5 kg) underwent PT including standardized femur fracture, lung contusion, liver laceration, hemorrhagic shock, subsequent resuscitation and surgical fracture fixation. Six animals served as controls (sham). After 72 h lung damage and inflammatory changes were assessed. LTB4 was determined in plasma before the experiment, immediately after trauma, and after 2, 4, 24 or 72 h. Bronchoalveolar lavage (BAL)-fluid was collected prior and after the experiment.
Results: Lung injury, local gene expression of IL-8, IL-1β, IL-10, IL-18 and PMN-infiltration into lungs increased significantly in PT compared with sham. Systemic LTB4 increased markedly in both groups 4 h after trauma. Compared with declined plasma LTB4 levels in sham, LTB4 increased further in PT after 72 h. Similar increase was observed in BAL-fluid after PT.
Conclusions: In a severe trauma model, sustained changes in terms of lung injury and inflammation are determined at day 3 post-trauma. Specifically, increased LTB4 in this porcine long-term model indicated a rapid inflammatory alteration both locally and systemically. The results support the concept of LTB4 as a biomarker for PC after severe trauma and lung contusion.
KW  - LTB4
KW  - Leukotriene
KW  - Inflammation
KW  - Biomarker
KW  - Trauma
KW  - Lung failure
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44712
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-447124
SN  - 1532-2823
SN  - 0952-3278
N1  - © 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
VL  - 127
SP  - 25
EP  - 31
PB  - Harcourt
CY  - Burlington, Mass.
ER  -