TY  - JOUR
A1  - Klemmt, Petra
A1  - Resch, Eduard
A1  - Smyrek, Isabell
A1  - Engels, Knut
A1  - Stelzer, Ernst H. K.
A1  - Starzinski-Powitz, Anna
T1  - Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile
T2  - Biology open
N2  - Shrew-1, also called AJAP1, is a transmembrane protein associated with E-cadherin-mediated adherence junctions and a putative tumor suppressor. Apart from its interaction with β-catenin and involvement in E-cadherin internalization, little structure or function information exists. Here we explored shrew-1 expression during postnatal differentiation of mammary gland as a model system. Immunohistological analyses with antibodies against either the extracellular or the cytoplasmic domains of shrew-1 consistently revealed the expression of full-length shrew-1 in myoepithelial cells, but only part of it in luminal cells. While shrew-1 localization remained unaltered in myoepithelial cells, nuclear localization occurred in luminal cells during lactation. Based on these observations, we identified two unknown shrew-1 transcript variants encoding N-terminally truncated proteins. The smallest shrew-1 protein lacks the extracellular domain and is most likely the only variant present in luminal cells. RNA analyses of human tissues confirmed that the novel transcript variants of shrew-1 exist in vivo and exhibit a differential tissue expression profile. We conclude that our findings are essential for the understanding and interpretation of future functional and interactome analyses of shrew-1 variants.
KW  - AJAP1
KW  - Mammary gland
KW  - Lactation
KW  - Splice variants
KW  - Adherence junctions
KW  - Tumor suppressor protein
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45138
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-451387
SN  - 2046-6390
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed
VL  - 5
IS  - 11
SP  - 1607
EP  - 1619
PB  - Company
CY  - Cambridge
ER  -