TY  - JOUR
A1  - Dörrbaum, Aline R.
A1  - Kochen, Lisa
A1  - Langer, Julian David
A1  - Schuman, Erin
T1  - Local and global influences on protein turnover in neurons and glia
T2  - eLife
N2  - Regulation of protein turnover allows cells to react to their environment and maintain homeostasis. Proteins can show different turnover rates in different tissue, but little is known about protein turnover in different brain cell types. We used dynamic SILAC to determine half-lives of over 5100 proteins in rat primary hippocampal cultures as well as in neuron-enriched and glia-enriched cultures ranging from <1 to >20 days. In contrast to synaptic proteins, membrane proteins were relatively shorter-lived and mitochondrial proteins were longer-lived compared to the population. Half-lives also correlate with protein functions and the dynamics of the complexes they are incorporated in. Proteins in glia possessed shorter half-lives than the same proteins in neurons. The presence of glia sped up or slowed down the turnover of neuronal proteins. Our results demonstrate that both the cell-type of origin as well as the nature of the extracellular environment have potent influences on protein turnover.
KW  - Research article
KW  - Cell biology
KW  - Neuroscience
KW  - Protein turnover
KW  - Hippocampus
KW  - Proteomics
KW  - Neuron-glia interactions
KW  - rat
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46872
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-468728
SN  - 2050-084X
N1  - Copyright Dörrbaum et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
VL  - 7
IS  - e34202
SP  - 1
EP  - 24
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -