TY  - JOUR
A1  - Vlijm, Rifka
A1  - Li, Xue
A1  - Panic, Marko
A1  - Rüthnick, Diana
A1  - Hata, Shoji
A1  - Herrmannsdörfer, Frank
A1  - Kuner, Thomas
A1  - Heilemann, Mike
A1  - Engelhardt, Johann
A1  - Hell, Stefan
A1  - Schiebel, Elmar
T1  - STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles
T2  - Proceedings of the National Academy of Sciences of the United States of America
N2  - The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structure of the centrosome linker. Here, we used stimulated emission depletion (STED) microscopy to show that each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers. Rootletin/CEP68 fibers originating from the two centrosomes form a web-like, interdigitating network, explaining the flexible nature of the centrosome linker. The rootletin/CEP68 filaments are repetitive and highly ordered. Staggered rootletin molecules (N-to-N and C-to-C) within the filaments are 75 nm apart. Rootletin binds CEP68 via its C-terminal spectrin repeat-containing region in 75-nm intervals. The N-to-C distance of two rootletin molecules is ∼35 to 40 nm, leading to an estimated minimal rootletin length of ∼110 nm. CEP68 is important in forming rootletin filaments that branch off centrioles and to modulate the thickness of rootletin fibers. Thus, the centrosome linker consists of a vast network of repeating rootletin units with C-Nap1 as ring organizer and CEP68 as filament modulator.
KW  - centrosome
KW  - centrosome linker
KW  - STED superresolution
KW  - rootletin
KW  - CEP68
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47822
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-478227
SN  - 0027-8424
SN  - 1091-6490
N1  - This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
VL  - 115
IS  - 10
SP  - E2246
EP  - E2253
PB  - National Acad. of Sciences
CY  - Washington, DC
ER  -