TY - JOUR A1 - Verboket, René A1 - Leiblein, Maximilian A1 - Seebach, Caroline A1 - Nau, Christoph A1 - Janko, Maren Carina A1 - Bellen, Marlene A1 - Bönig, Halvard-Björn A1 - Henrich, Dirk A1 - Marzi, Ingo T1 - Autologous cell-based therapy for treatment of large bone defects : from bench to bedside T2 - European journal of trauma and emergency surgery N2 - Objectives: Reconstruction of long segmental bone defects is demanding for patients and surgeons, and associated with long-term treatment periods and substantial complication rates in addition to high costs. While defects up to 4–5 cm length might be filled up with autologous bone graft, heterologous bone from cadavers, or artificial bone graft substitutes, current options to reconstruct bone defects greater than 5 cm consist of either vascularized free bone transfers, the Masquelet technique or the Ilizarov distraction osteogenesis. Alternatively, autologous cell transplantation is an encouraging treatment option for large bone defects as it eliminates problems such as limited autologous bone availability, allogenic bone immunogenicity, and donor-site morbidity, and might be used for stabilizing loose alloplastic implants. Methods: The authors show different cell therapies without expansion in culture, with ex vivo expansion and cell therapy in local bone defects, bone healing and osteonecrosis. Different kinds of cells and scaffolds investigated in our group as well as in vivo transfer studies and BMC used in clinical phase I and IIa clinical trials of our group are shown. Results: Our research history demonstrated the great potential of various stem cell species to support bone defect healing. It was clearly shown that the combination of different cell types is superior to approaches using single cell types. We further demonstrate that it is feasible to translate preclinically developed protocols from in vitro to in vivo experiments and follow positive convincing results into a clinical setting to use autologous stem cells to support bone healing. KW - Bone defect KW - Cell therapy KW - Stem cells KW - BMC KW - Bone marrow mononuclear cells KW - Regeneration Y1 - 2018 UR - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48691 UR - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-486917 SN - 1863-9941 SN - 1863-9933 N1 - Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. VL - 44 IS - 5 SP - 649 EP - 665 PB - Springer Medizin CY - Heidelberg ER -