TY  - JOUR
A1  - Henrich, Dirk
A1  - Verboket, René
A1  - Schaible, Alexander
A1  - Kontradowitz, Kerstin
A1  - Oppermann, Elsie
A1  - Brune, Jan C.
A1  - Nau, Christoph
A1  - Meier, Simon
A1  - Bönig, Halvard-Björn
A1  - Marzi, Ingo
A1  - Seebach, Caroline
T1  - Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro
T2  - BioMed research international
N2  - Bone marrow mononuclear cells (BMCs) are suitable for bone tissue engineering. Comparative data regarding the needs of BMC for the adhesion on biomaterials and biocompatibility to various biomaterials are lacking to a large extent. Therefore, we evaluated whether a surface coating would enhance BMC adhesion and analyze the biocompatibility of three different kinds of biomaterials. BMCs were purified from human bone marrow aspirate samples. Beta tricalcium phosphate (β-TCP, without coating or coated with fibronectin or human plasma), demineralized bone matrix (DBM), and bovine cancellous bone (BS) were assessed. Seeding efficacy on β-TCP was 95% regardless of the surface coating. BMC demonstrated a significantly increased initial adhesion on DBM and β-TCP compared to BS. On day 14, metabolic activity was significantly increased in BMC seeded on DBM in comparison to BMC seeded on BS. Likewise increased VEGF-synthesis was observed on day 2 in BMC seeded on DBM when compared to BMC seeded on BS. The seeding efficacy of BMC on uncoated biomaterials is generally high although there are differences between these biomaterials. Beta-TCP and DBM were similar and both superior to BS, suggesting either as suitable materials for spatial restriction of BMC used for regenerative medicine purposes in vivo.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/50807
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-508079
SN  - 2314-6141
SN  - 2314-6133
N1  - Copyright © 2015 Dirk Henrich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
N1  - Corrigendum erschienen in: BioMed Research International, 2015, Art. 325203, doi:10.1155/2015/325203
VL  - 2015
IS  - Art. 762407
SP  - 1
EP  - 12
PB  - Hindawi
CY  - New York [u. a.]
ER  -