TY  - JOUR
A1  - Kreis, Nina-Naomi
A1  - Friemel, Alexandra
A1  - Ritter, Andreas Hans
A1  - Roth, Susanne
A1  - Rolle, Udo
A1  - Louwen, Frank
A1  - Yuan, Juping
T1  - Function of p21 (Cip1/Waf1/CDKN1A) in migration and invasion of cancer and trophoblastic cells
T2  - Cancers
N2  - Tumor progression and pregnancy have several features in common. Tumor cells and placental trophoblasts share many signaling pathways involved in migration and invasion. Preeclampsia, associated with impaired differentiation and migration of trophoblastic cells, is an unpredictable and unpreventable disease leading to maternal and perinatal mortality and morbidity. Like in tumor cells, most pathways, in which p21 is involved, are deregulated in trophoblasts of preeclamptic placentas. The aim of the present study was to enlighten p21’s role in tumorigenic choriocarcinoma and trophoblastic cell lines. We show that knockdown of p21 induces defects in chromosome movement during mitosis, though hardly affecting proliferation and cell cycle distribution. Moreover, suppression of p21 compromises the migration and invasion capability of various trophoblastic and cancer cell lines mediated by, at least partially, a reduction of the extracellular signal-regulated kinase 3, identified using transcriptome-wide profiling, real-time PCR, and Western blot. Further analyses show that downregulation of p21 is associated with reduced matrix metalloproteinase 2 and tissue inhibitor of metalloproteinases 2. This work evinces that p21 is involved in chromosome movement during mitosis as well as in the motility and invasion capacity of trophoblastic and cancer cell lines.
KW  - p21
KW  - CDKN1A
KW  - trophoblastic cell lines
KW  - invasion
KW  - ERK3/MAPK6
KW  - MMP2
KW  - TIMP2
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51264
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-512646
SN  - 2072-6694
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 11
IS  - 7, Art. 989
SP  - 1
EP  - 17
PB  - MDPI
CY  - Basel
ER  -