TY  - JOUR
A1  - Wang, Mantian
A1  - Hou, Jingyi
A1  - Müller-McNicoll, Michaela
A1  - Chen, Wei
A1  - Schuman, Erin
T1  - Long and repeat-rich intronic sequences favor circular rna formation under conditions of reduced spliceosome activity
T2  - iScience
N2  - Circular RNAs (circRNAs), an important class of regulatory RNAs, have been shown to be the most prevalent in the brain compared with other tissues. However the processes governing their biogenesis in neurons are still elusive. Moreover, little is known about whether and how different biogenesis factors work in synchrony to generate neuronal circRNAs. To address this question, we pharmacologically inhibited the spliceosome and profiled rat neuronal circRNAs using RNA sequencing. We identified over 100 circRNAs that were up-regulated and a few circRNAs that were down-regulated upon spliceosome inhibition. Bioinformatic analysis revealed that up-regulated circRNAs possess significantly longer flanking introns compared with the un-changed circRNA population. Moreover, the flanking introns of up-regulated circRNAs harbor a higher number of distinct repeat sequences and more reverse complementary motifs compared with the unchanged circRNAs. Taken together, our data demonstrate that the biogenesis of circRNAs containing distinct intronic features becomes favored under conditions of limited spliceosome activity.
KW  - Biological Sciences
KW  - Molecular Biology
KW  - Cell Biology
KW  - Omics
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51426
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-514269
SN  - 2589-0042
N1  - This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
VL  - 20
SP  - 237
EP  - 247
PB  - Elsevier
CY  - Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis
ER  -