TY  - JOUR
A1  - Bhavsar, Mit Balvantray
A1  - Leppik, Liudmila
A1  - Costa Oliveira, Karla Mychellyne
A1  - Barker, John Howard
T1  - Role of bioelectricity during cell proliferation in different cell types
T2  - Frontiers in bioengineering and biotechnology
N2  - Most living organisms possess varying degrees of regenerative capabilities but how these regenerative processes are controlled is still poorly understood. Naturally occurring bioelectric voltages (like Vmem) are thought to be playing instructive role in tissue regeneration, as well as embryonic development. The different distribution of ions on the either side of the cell membrane results in intra- and extra-cellular voltage differences, known as membrane potential or Vmem. The relationship between Vmem and cell physiology is conserved in a wide range of cell types and suggests that Vmem regulation is a fundamental control mechanism for regeneration related processes e.g., proliferation and differentiation. In the present study we measured Vmem in three different cell types (human osteogenic sarcoma cell line (OSC), rat bone marrow derived mesenchymal stem cells (BM-MSC), and rat dermal fibroblasts) and characterized the relationship between their Vmem and proliferation. In order to find out if Vmem controls proliferation, or visa-versa, we blocked and then unblocked Na+/K+-exchanging ATPase using ouabain and measured the proliferation. Our results demonstrate that Vmem can be pharmacologically manipulated to control proliferation in certain cell types like BM-MSC. Taken together, it is clear that control of bioelectrical properties in non-excitable cells could prove to be potentially a useful tool in regenerative medicine efforts.
KW  - membrane potential
KW  - Vmem
KW  - cell proliferation
KW  - ouabain
KW  - mesenchymal stem cell
KW  - osteogenic sarcoma cells
KW  - fibroblasts
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55055
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-550558
SN  - 2296-4185
VL  - 8
IS  - Art. 603
SP  - 1
EP  - 9
PB  - Frontiers Media S.A.
CY  - Lausanne
ER  -