TY  - JOUR
A1  - Jiang, Bingfu
A1  - Wen, Xingjian
A1  - Wu, Qingyan
A1  - Bender, Daniela
A1  - Carra, Gert
A1  - Basic, Michael
A1  - Kubesch, Alica
A1  - Peiffer, Kai-Henrik
A1  - Boller, Klaus
A1  - Hildt, Eberhard
T1  - The N-terminus makes the difference: impact of genotype-specific disparities in the N-terminal part of the hepatitis B virus large surface protein on morphogenesis of viral and subviral particles
T2  - Cells
N2  - The N-terminus of the hepatitis B virus (HBV) large surface protein (LHB) differs with respect to genotypes. Compared to the amino terminus of genotype (Gt)D, in GtA, GtB and GtC, an additional identical 11 amino acids (aa) are found, while GtE and GtG share another similar 10 aa. Variants of GtB and GtC affecting this N-terminal part are associated with hepatoma formation. Deletion of these amino-terminal 11 aa in GtA reduces the amount of LHBs and changes subcellular accumulation (GtA-like pattern) to a dispersed distribution (GtD-like pattern). Vice versa, the fusion of the GtA-derived N-terminal 11 aa to GtD causes a GtA-like phenotype. However, insertion of the corresponding GtE-derived 10 aa to GtD has no effect. Deletion of these 11aa decreases filament size while neither the number of released viral genomes nor virion size and infectivity are affected. A negative regulatory element (aa 2–8) and a dominant positive regulatory element (aa 9–11) affecting the amount of LHBs were identified. The fusion of this motif to eGFP revealed that the effect on protein amount and subcellular distribution is not restricted to LHBs. These data identify a novel region in the N-terminus of LHBs affecting the amount and subcellular distribution of LHBs and identify release-promoting and -inhibiting aa residues within this motive.
KW  - HBV surface protein
KW  - PreS1 deletion
KW  - HBV filaments
KW  - HBV genotypes
KW  - morphogenesis
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55590
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-555906
SN  - 2073-4409
VL  - 9
IS  - (8), 1898
PB  - MDPI
CY  - Basel
ER  -