TY  - JOUR
A1  - Blankenbach, Kira Vanessa
A1  - Claas, Ralf Frederik
A1  - Aster, Natalie Judith
A1  - Spohner, Anna Katharina
A1  - Trautmann, Sandra
A1  - Ferreirós Bouzas, Nerea
A1  - Black, Justin L.
A1  - Tesmer, John J. G.
A1  - Offermanns, Stefan
A1  - Wieland, Thomas
A1  - Meyer zu Heringdorf, Dagmar
T1  - Dissecting Gq/11-mediated plasma membrane translocation of sphingosine kinase-1
T2  - Cells
N2  - Diverse extracellular signals induce plasma membrane translocation of sphingosine kinase-1 (SphK1), thereby enabling inside-out signaling of sphingosine-1-phosphate. We have shown before that Gq-coupled receptors and constitutively active Gαq/11 specifically induced a rapid and long-lasting SphK1 translocation, independently of canonical Gq/phospholipase C (PLC) signaling. Here, we further characterized Gq/11 regulation of SphK1. SphK1 translocation by the M3 receptor in HEK-293 cells was delayed by expression of catalytically inactive G-protein-coupled receptor kinase-2, p63Rho guanine nucleotide exchange factor (p63RhoGEF), and catalytically inactive PLCβ3, but accelerated by wild-type PLCβ3 and the PLCδ PH domain. Both wild-type SphK1 and catalytically inactive SphK1-G82D reduced M3 receptor-stimulated inositol phosphate production, suggesting competition at Gαq. Embryonic fibroblasts from Gαq/11 double-deficient mice were used to show that amino acids W263 and T257 of Gαq, which interact directly with PLCβ3 and p63RhoGEF, were important for bradykinin B2 receptor-induced SphK1 translocation. Finally, an AIXXPL motif was identified in vertebrate SphK1 (positions 100–105 in human SphK1a), which resembles the Gαq binding motif, ALXXPI, in PLCβ and p63RhoGEF. After M3 receptor stimulation, SphK1-A100E-I101E and SphK1-P104A-L105A translocated in only 25% and 56% of cells, respectively, and translocation efficiency was significantly reduced. The data suggest that both the AIXXPL motif and currently unknown consequences of PLCβ/PLCδ(PH) expression are important for regulation of SphK1 by Gq/11.
KW  - sphingosine kinase
KW  - sphingosine-1-phosphate
KW  - G-protein-coupled receptors
KW  - Gαq/11
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56420
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-564203
SN  - 2073-4409
N1  - © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 9
IS  - art. 2201
SP  - 1
EP  - 17
PB  - MDPI
CY  - Basel
ER  -