TY  - JOUR
A1  - Heidepriem, Jasmin
A1  - Krähling, Verena
A1  - Dahlke, Christine
A1  - Wolf, Timo
A1  - Klein, Florian
A1  - Addo, Marylyn M.
A1  - Becker, Stephan
A1  - Löffler, Felix F.
T1  - Epitopes of naturally acquired and vaccine‐induced anti‐ebola virus glycoprotein antibodies in single amino acid resolution
T2  - Biotechnology journal
N2  - The Ebola virus (EBOV) can cause severe infections in humans, leading to a fatal outcome in a high percentage of cases. Neutralizing antibodies against the EBOV surface glycoprotein (GP) can prevent infections, demonstrating a straightforward way for an efficient vaccination strategy. Meanwhile, many different anti‐EBOV antibodies have been identified, whereas the exact binding epitopes are often unknown. Here, the analysis of serum samples from an EBOV vaccine trial with the recombinant vesicular stomatitis virus‐Zaire ebolavirus (rVSV‐ZEBOV) and an Ebola virus disease survivor, using high‐density peptide arrays, is presented. In this proof‐of‐principle study, distinct IgG and IgM antibodies binding to different epitopes of EBOV GP is detected: By mapping the whole GP as overlapping peptide fragments, new epitopes and confirmed epitopes from the literature are found. Furthermore, the highly selective binding epitope of a neutralizing monoclonal anti‐EBOV GP antibody could be validated. This shows that peptide arrays can be a valuable tool to study the humoral immune response to vaccines in patients and to support Ebola vaccine development.
KW  - Ebola virus
KW  - epitope mapping
KW  - human sera
KW  - neutralizing antibodies
KW  - recombinant vesicular stomatitis virus‐Zaire ebolavirus
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57069
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-570695
SN  - 1860-7314
VL  - 15
IS  - 9
PB  - Wiley‐VCH Verlag GmbH & Co. KGaA
CY  - Weinheim
ER  -