TY  - JOUR
A1  - Knuth, Anne-Kathrin
A1  - Huard, Arnaud
A1  - Naeem, Zumer
A1  - Rappl, Peter
A1  - Bauer, Rebekka
A1  - Mota, Ana Carolina
A1  - Schmid, Tobias
A1  - Fleming, Ingrid
A1  - Brüne, Bernhard
A1  - Fulda, Simone
A1  - Weigert, Andreas
T1  - Apoptotic cells induce proliferation of peritoneal macrophages
T2  - International journal of molecular sciences
N2  - The interaction of macrophages with apoptotic cells is required for efficient resolution of inflammation. While apoptotic cell removal prevents inflammation due to secondary necrosis, it also alters the macrophage phenotype to hinder further inflammatory reactions. The interaction between apoptotic cells and macrophages is often studied by chemical or biological induction of apoptosis, which may introduce artifacts by affecting the macrophages as well and/or triggering unrelated signaling pathways. Here, we set up a pure cell death system in which NIH 3T3 cells expressing dimerizable Caspase-8 were co-cultured with peritoneal macrophages in a transwell system. Phenotype changes in macrophages induced by apoptotic cells were evaluated by RNA sequencing, which revealed an unexpectedly dominant impact on macrophage proliferation. This was confirmed in functional assays with primary peritoneal macrophages and IC-21 macrophages. Moreover, inhibition of apoptosis during Zymosan-induced peritonitis in mice decreased mRNA levels of cell cycle mediators in peritoneal macrophages. Proliferation of macrophages in response to apoptotic cells may be important to increase macrophage numbers in order to allow efficient clearance and resolution of inflammation.
KW  - apoptosis
KW  - peritoneal macrophages
KW  - RNA sequencing
KW  - Zymosan-induced peritonitis
KW  - proliferation
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/60993
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-609937
SN  - 1422-0067
VL  - 22
IS  - 5, art. 2230
SP  - 1
EP  - 17
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -