TY  - JOUR
A1  - Gutwein, Paul
A1  - Schramme, Anja
A1  - Abdel-Bakky, Mohamed Sadek
A1  - Doberstein, Kai
A1  - Hauser, Ingeborg A.
A1  - Ludwig, Andreas
A1  - Altevogt, Peter
A1  - Gauer, Stefan
A1  - Hillmann, Anja
A1  - Weide, Thomas
A1  - Jespersen, Christine
A1  - Eberhardt, Wolfgang
A1  - Pfeilschifter, Josef
T1  - ADAM10 is expressed in human podocytes and found in urinary vesicles of patients with glomerular kidney diseases
T2  - Journal of biomedical science
N2  - Background: The importance of the Notch signaling in the development of glomerular diseases has been recently described. Therefore we analyzed in podocytes the expression and activity of ADAM10, one important component of the Notch signaling complex. Methods: By Western blot, immunofluorescence and immunohistochemistry analysis we characterized the expression of ADAM10 in human podocytes, human urine and human renal tissue. Results: We present evidence, that differentiated human podocytes possessed increased amounts of mature ADAM10 and released elevated levels of L1 adhesion molecule, one well known substrate of ADAM10. By using specific siRNA and metalloproteinase inhibitors we demonstrate that ADAM10 is involved in the cleavage of L1 in human podocytes. Injury of podocytes enhanced the ADAM10 mediated cleavage of L1. In addition, we detected ADAM10 in urinary podocytes from patients with kidney diseases and in tissue sections of normal human kidney. Finally, we found elevated levels of ADAM10 in urinary vesicles of patients with glomerular kidney diseases. Conclusions: The activity of ADAM10 in human podocytes may play an important role in the development of glomerular kidney diseases.
Y1  - 2010
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7598
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-73769
SN  - 1423-0127
SN  - 1021-7770
N1  - © 2010 Gutwein et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 17
IS  - 1, Art. 3
SP  - 1
EP  - 9
PB  - BioMed Central
CY  - London
ER  -