TY  - JOUR
A1  - Steinert, Hannah
A1  - Sochor, Florian
A1  - Wacker, Anna
A1  - Buck, Janina
A1  - Helmling, Christina
A1  - Hiller, Fabian
A1  - Keyhani, Sara
A1  - Noeske, Jonas
A1  - Grimm, Steffen
A1  - Rudolph, Martin M.
A1  - Keller, Heiko
A1  - Mooney, Rachel Anne
A1  - Dick, Rolf van
A1  - Süß, Beatrix
A1  - Fürtig, Boris
A1  - Wöhnert, Jens
A1  - Schwalbe, Harald
T1  - Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation
T2  - eLife
N2  - In bacteria, the regulation of gene expression by cis-acting transcriptional riboswitches located in the 5'-untranslated regions of messenger RNA requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational refolding. Ligand binding to the aptamer domain of the riboswitch induces premature termination of the mRNA synthesis of ligand-associated genes due to the coupled formation of 3'-structural elements acting as terminators. To date, there has been no high resolution structural description of the concerted process of synthesis and ligand-induced restructuring of the regulatory RNA element. Here, we show that for the guanine-sensing xpt-pbuX riboswitch from Bacillus subtilis, the conformation of the full-length transcripts is static: it exclusively populates the functional off-state but cannot switch to the on-state, regardless of the presence or absence of ligand. We show that only the combined matching of transcription rates and ligand binding enables transcription intermediates to undergo ligand-dependent conformational refolding.
KW  - B. subtilis
KW  - E. coli
KW  - RNA
KW  - biochemistry
KW  - biophysics
KW  - folding
KW  - kinetics
KW  - meta-stable structures
KW  - riboswitches
KW  - structural biology
KW  - transcription
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43906
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-439063
SN  - 2050-084X
N1  - Copyright Steinert et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
VL  - 6
IS  - e21297
SP  - 1
EP  - 18
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -