TY  - JOUR
A1  - Zhao, Lei
A1  - Vo, Tien Duy
A1  - Kaiser, Marcel
A1  - Bode, Helge Björn
T1  - Phototemtide A, a cyclic lipopeptide heterologously expressed from Photorhabdus temperata Meg1, shows selective antiprotozoal activity
T2  - ChemBioChem
N2  - A new cyclic lipopeptide, phototemtide A (1), was isolated from Escherichia coli expressing the biosynthetic gene cluster pttABC from Photorhabdus temperata Meg1. The structure of 1 was elucidated by HR‐ESI‐MS and NMR experiments. The absolute configurations of amino acids and 3‐hydroxyoctanoic acid in 1 were determined by using the advanced Marfey's method and comparison after total synthesis of 1, respectively. Additionally, three new minor derivatives, phototemtides B–D (2–4), were identified by detailed HPLC–MS analysis. Phototemtide A (1) showed weak antiprotozoal activity against Plasmodium falciparum, with an IC50 value of 9.8 μm. The biosynthesis of phototemtides A–D (1–4) was also proposed.
KW  - biosynthesis
KW  - peptides
KW  - Photorhabdus
KW  - structure elucidation
KW  - total synthesis
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57042
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-570424
SN  - 1439-7633
VL  - 21.2020
IS  - 9
SP  - 1288
EP  - 1292
PB  - Wiley-VCH
CY  - Weinheim
ER  -