TY  - JOUR
A1  - Weidensdorfer, Marko
A1  - Ishikawa, Masahito
A1  - Hori, Katsutoshi
A1  - Linke, Dirk
A1  - Djahanschiri, Bardya
A1  - Iruegas, Ruben
A1  - Ebersberger, Ingo
A1  - Riedel-Christ, Sara
A1  - Enders, Giulia
A1  - Leukert, Laura
A1  - Kraiczy, Peter
A1  - Rothweiler, Florian
A1  - Cinatl, Jindrich
A1  - Berger, Jürgen
A1  - Hipp, Katharina
A1  - Kempf, Volkhard A. J.
A1  - Göttig, Stephan
T1  - The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii
T2  - Virulence
N2  - Acinetobacter baumannii is a Gram-negative pathogen that causes a multitude of nosocomial infections. The Acinetobacter trimeric autotransporter adhesin (Ata) belongs to the superfamily of trimeric autotransporter adhesins which are important virulence factors in many Gram-negative species. Phylogenetic profiling revealed that ata is present in 78% of all sequenced A. baumannii isolates but only in 2% of the closely related species A. calcoaceticus and A. pittii. Employing a markerless ata deletion mutant of A. baumannii ATCC 19606 we show that adhesion to and invasion into human endothelial and epithelial cells depend on Ata. Infection of primary human umbilical cord vein endothelial cells (HUVECs) with A. baumannii led to the secretion of interleukin (IL)-6 and IL-8 in a time- and Ata-dependent manner. Furthermore, infection of HUVECs by WT A. baumannii was associated with higher rates of apoptosis via activation of caspases-3 and caspase-7, but not necrosis, in comparison to ∆ata. Ata deletion mutants were furthermore attenuated in their ability to kill larvae of Galleria mellonella and to survive in larvae when injected at sublethal doses. This indicates that Ata is an important multifunctional virulence factor in A. baumannii that mediates adhesion and invasion, induces apoptosis and contributes to pathogenicity in vivo.
KW  - Adhesion
KW  - endothelial cells
KW  - host cell response
KW  - HUVEC
KW  - Galleria mellonella
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/49286
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-492860
SN  - 2150-5608
SN  - 2150-5594
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 10
IS  - 1
SP  - 68
EP  - 81
PB  - Landes Bioscience
CY  - Austin, Tex.
ER  -