TY  - JOUR
A1  - Stoev, Pavel
A1  - Komerički, Ana
A1  - Akkari, Nesrine
A1  - Liu, Shanlin
A1  - Zhou, Xin
A1  - Weigand, Alexander
A1  - Hostens, Jeroen
A1  - Hunter, Christopher I.
A1  - Edmunds, Scott C.
A1  - Porco, David
A1  - Zapparoli, Marzio
A1  - Georgiev, Teodor
A1  - Mietchen, Daniel
A1  - Roberts, David
A1  - Faulwetter, Sarah
A1  - Smith, Vincent
A1  - Penev, Lyubomir
T1  - Eupolybothrus cavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae): the first eukaryotic species description combining transcriptomic, DNA barcoding and micro-CT imaging data
T2  - Biodiversity Data Journal
N2  - We demonstrate how a classical taxonomic description of a new species can be enhanced by applying new generation molecular methods, and novel computing and imaging technologies. A cave-dwelling centipede, Eupolybothrus cavernicolus Komerički & Stoev sp. n. (Chilopoda: Lithobiomorpha: Lithobiidae), found in a remote karst region in Knin, Croatia, is the first eukaryotic species for which, in addition to the traditional morphological description, we provide a fully sequenced transcriptome, a DNA barcode, detailed anatomical X-ray microtomography (micro-CT) scans, and a movie of the living specimen to document important traits of its ex-situ behaviour. By employing micro-CT scanning in a new species for the first time, we create a high-resolution morphological and anatomical dataset that allows virtual reconstructions of the specimen and subsequent interactive manipulation to test the recently introduced ‘cybertype’ notion. In addition, the transcriptome was recorded with a total of 67,785 scaffolds, having an average length of 812 bp and N50 of 1,448 bp (see GigaDB). Subsequent annotation of 22,866 scaffolds was conducted by tracing homologs against current available databases, including Nr, SwissProt and COG. This pilot project illustrates a workflow of producing, storing, publishing and disseminating large data sets associated with a description of a new taxon. All data have been deposited in publicly accessible repositories, such as GigaScience GigaDB, NCBI, BOLD, Morphbank and Morphosource, and the respective open licenses used ensure their accessibility and re-usability.
KW  - Cybertaxonomy
KW  - gene sequence data
KW  - micro-CT
KW  - data integration
KW  - molecular systematics
KW  - caves
KW  - Croatia
KW  - biospeleology
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43892
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-438921
SN  - 1314-2828
N1  - © Stoev P et al.. This is an open access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC-BY), which
permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 1
IS  - e1013
PB  - Pensoft Publishers
CY  - Sofia
ER  -