TY  - JOUR
A1  - Henkel, Vanessa
A1  - Schürmanns, Lea
A1  - Brunner, Miriam
A1  - Hamann, Andrea
A1  - Osiewacz, Heinz D.
T1  - Role of sorting nexin PaATG24 in autophagy, aging and development of Podospora anserina
T2  - Mechanisms of ageing and development
N2  - Sorting nexins are a conserved protein family involved in vesicle transport, membrane trafficking and protein sorting. The sorting nexin ATG24/SNX4 has been demonstrated to be involved in different autophagy pathways and in endosomal trafficking. However, its impact on cellular quality control and on aging and development is still elusive. Here we report studies analyzing the function of PaATG24 in the aging model Podospora anserina. Ablation of PaATG24 leads to a reduced growth rate, infertility, and to a pronounced lifespan reduction. These characteristics are accompanied by alterations of the morphology and size distribution of vacuoles and severe impairments in non-selective and selective autophagy of peroxisomes (pexophagy) and mitochondria (mitophagy). While general autophagy and pexophagy are almost completely blocked, a PaATG24-independent form of mitophagy is induced during aging. In the ΔPaAtg24 mutant a strong accumulation of peroxisomes occurs while mitochondrial abundance is only slightly increased. These mitochondria are partially affected in function. Most strikingly, although some PaATG24-independent mitophagy exists, it appears that this is not sufficient to remove dysfunctional mitochondria efficiently enough to prevent premature aging. Overall our data emphasize the key role of mitochondria in aging and of mitophagy in quality control to keep a population of “healthy” mitochondria during aging.
KW  - Podospora anserina
KW  - Aging
KW  - Sorting nexins
KW  - Autophagy
KW  - Mitophagy
KW  - Pexophagy
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52884
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-528846
SN  - 1872-6216
SN  - 0047-6374
N1  - © 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
VL  - 186
IS  - Art. 111211
SP  - 1
EP  - 11
PB  - Elsevier Science
CY  - Amsterdam [u. a.]
ER  -