TY  - JOUR
A1  - Bullmann, Lars
A1  - Haarmann, Raimund
A1  - Mirus, Oliver
A1  - Bredemeier, Rolf
A1  - Hempel, Franziska
A1  - Maier, Uwe G.
A1  - Schleiff, Enrico
T1  - Filling the gap, evolutionarily conserved Omp85 in plastids of chromalveolates
T2  - Journal of biological chemistry
N2  - Chromalveolates are a diverse group of protists that include many ecologically and medically relevant organisms such as diatoms and apicomplexan parasites. They possess plastids generally surrounded by four membranes, which evolved by engulfment of a red alga. Today, most plastid proteins must be imported, but many aspects of protein import into complex plastids are still cryptic. In particular, how proteins cross the third outermost membrane has remained unexplained. We identified a protein in the third outermost membrane of the diatom Phaeodactylum tricornutum with properties comparable to those of the Omp85 family. We demonstrate that the targeting route of P. tricornutum Omp85 parallels that of the translocation channel of the outer envelope membrane of chloroplasts, Toc75. In addition, the electrophysiological properties are similar to those of the Omp85 proteins involved in protein translocation. This supports the hypothesis that P. tricornutum Omp85 is involved in precursor protein translocation, which would close a gap in the fundamental understanding of the evolutionary origin and function of protein import in secondary plastids.
KW  - Evolution/Protein
KW  - Membrane/Proteins
KW  - Protein/Sorting
KW  - Protein/Targeting
KW  - Protein/Translocation
KW  - Subcellular Organelles
KW  - Transport
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76487
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-764874
SN  - 0021-9258
VL  - 285.2010
IS  - 9
SP  - 6848
EP  - 6856
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -