TY  - JOUR
A1  - Tsuda, Kengo
A1  - Kuwasako, Kanako
A1  - Takahashi, Mari
A1  - Someya, Tatsuhiko
A1  - Inoue, Makoto
A1  - Terada, Takaho
A1  - Kobayashi, Naohiro
A1  - Shirouzu, Mikako
A1  - Kigawa, Takanori
A1  - Tanaka, Akiko
A1  - Sugano, Sumio
A1  - Güntert, Peter
A1  - Muto, Yutaka
A1  - Yokoyama, Shigeyuki
T1  - Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3
T2  - Nucleic acids research
N2  - The CUG-binding protein 1 (CUG-BP1) is a member of the CUG-BP1 and ETR-like factors (CELF) family or the Bruno-like family and is involved in the control of splicing, translation and mRNA degradation. Several target RNA sequences of CUG-BP1 have been predicted, such as the CUG triplet repeat, the GU-rich sequences and the AU-rich element of nuclear pre-mRNAs and/or cytoplasmic mRNA. CUG-BP1 has three RNA-recognition motifs (RRMs), among which the third RRM (RRM3) can bind to the target RNAs on its own. In this study, we solved the solution structure of the CUG-BP1 RRM3 by hetero-nuclear NMR spectroscopy. The CUG-BP1 RRM3 exhibited a noncanonical RRM fold, with the four-stranded b-sheet surface tightly associated with the N-terminal extension. Furthermore, we determined the solution structure of the CUG-BP1 RRM3 in the complex with (UG)3 RNA, and discovered that the UGU trinucleotide is specifically recognized through extensive stacking interactions and hydrogen bonds within the pocket formed by the b-sheet surface and the N-terminal extension. This study revealed the unique mechanism that enables the CUG-BP1 RRM3 to discriminate the short RNA segment from other sequences, thus providing the molecular basis for the comprehension of the role of the RRM3s in the CELF/Bruno-like family.
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/6707
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-68081
SN  - 1362-4962
SN  - 0305-1048
N1  - © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 37
IS  - 15
SP  - 5151
EP  - 5166
PB  - Oxford Univ. Press
CY  - Oxford
ER  -