TY  - JOUR
A1  - Kohli, Aneesha
A1  - Sauerhering, Lucie
A1  - Fehling, Sarah Katharina
A1  - Klann, Kevin
A1  - Geiger, Helmut
A1  - Becker, Stephan
A1  - Koch, Benjamin Florian
A1  - Baer, Patrick C.
A1  - Strecker, Thomas
A1  - Münch, Christian
T1  - Proteomic landscape of SARS-CoV-2 and MERS-CoVinfected primary human renal epithelial cells
T2  - Life Science Alliance
N2  - Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type–specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection–induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type–specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.
KW  - Cell Biology
KW  - Microbiology, Virology & Host Pathogen Interaction
KW  - Systems & Computational Biology
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83839
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-838396
SN  - 2575-1077
N1  - The LC-MS/MS proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE (102) partner repository with the dataset identifier: PXD024398.
VL  - 5
IS  - 5, art. e202201371
SP  - 1
EP  - 19
PB  - EMBO Press
CY  - Heidelberg
ER  -