TY  - JOUR
A1  - Grewe, Felix
A1  - Lagostina, Elisa
A1  - Wu, Huini
A1  - Printzen, Christian
A1  - Lumbsch, Helge Thorsten
T1  - Population genomic analyses of RAD sequences resolves the phylogenetic relationship of the lichen-forming fungal species Usnea antarctica and Usnea aurantiacoatra
T2  - MycoKeys
N2  - Neuropogonoid species in the lichen-forming fungal genus Usnea exhibit great morphological variation that can be misleading for delimitation of species. We specifically focused on the species delimitation of two closely-related, predominantly Antarctic species differing in the reproductive mode and representing a so-called species pair: the asexual U. antarctica and the sexual U. aurantiacoatra. Previous studies have revealed contradicting results. While multi-locus studies based on DNA sequence data provided evidence that these two taxa might be conspecific, microsatellite data suggested they represent distinct lineages. By using RADseq, we generated thousands of homologous markers to build a robust phylogeny of the two species. Furthermore, we successfully implemented these data in fine-scale population genomic analyses such as DAPC and fineRADstructure. Both Usnea species are readily delimited in phylogenetic inferences and, therefore, the hypothesis that both species are conspecific was rejected. Population genomic analyses also strongly confirmed separated genomes and, additionally, showed different levels of co-ancestry and substructure within each species. Lower co-ancestry in the asexual U. antarctica than in the sexual U. aurantiacoatra may be derived from a wider distributional range of the former species. Our results demonstrate the utility of this RADseq method in tracing population dynamics of lichens in future analyses.
KW  - Antarctica
KW  - Ascomycota
KW  - lichens
KW  - Parmeliaceae
KW  - phylogeny
KW  - RADseq
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48608
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-486081
SN  - 1314-4057
SN  - 1314-4049
N1  - Copyright Felix Grewe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 43
SP  - 91
EP  - 113
PB  - Pensoft Publishers
CY  - Washington, DC [u. a.]
ER  -