TY  - JOUR
A1  - Müller, Mareike
A1  - Merz, Felicitas
A1  - Durante, Marco
A1  - Seifert, Volker
A1  - Rieger, Johannes
A1  - Mittelbronn, Michel Guy André
A1  - Harter, Patrick Nikolaus
A1  - Mishustin, Igor N.
A1  - Pshenichnov, Igor A.
A1  - Rödel, Franz
A1  - Stöcker, Horst
A1  - Schopow, Kosta
A1  - Dehghani, Faramarz
A1  - Taucher-Scholz, Gisela
A1  - Bechmann, Ingo
T1  - Heavy ions and X-rays in brain tumor treatment : a comparison of their biological effects on tissue slice cultures
T2  - PTCOG 48. Meeting of the Particle Therapy Co-Operative Group. Heidelberg, 28.09.-03.10.2009
N2  - Background: In this interdisciplinary project, the biological effects of heavy ions are compared to those of X-rays using tissue slice culture preparations from rodents and humans. Advantages of this biological model are the conservation of an organotypic environment and the independency from genetic immortalization strategies used to generate cell lines. Its open access allows easy treatment and observation via live-imaging microscopy. Materials and methods: Rat brains and human brain tumor tissue are cut into 300 micro m thick tissue slices. These slices are cultivated using a membrane-based culture system and kept in an incubator at 37°C until treatment. The slices are treated with X-rays at the radiation facility of the University Hospital in Frankfurt at doses of up to 40 Gy. The heavy ion irradiations were performed at the UNILAC facility at GSI with different ions of 11.4 A MeV and fluences ranging from 0.5–10 x 106 particles/cm². Using 3D-confocal microscopy, cell-death and immune cell activation of the irradiated slices are analyzed. Planning of the irradiation experiments is done with simulation programs developed at GSI and FIAS. Results: After receiving a single application of either X-rays or heavy ions, slices were kept in culture for up to 9d post irradiation. DNA damage was visualized using gamma H2AXstaining. Here, a dose-dependent increase and time-dependent decrease could clearly be observed for the X-ray irradiation. Slices irradiated with heavy ions showed less gamma H2AX-positive cells distributed evenly throughout the slice, even though particles were calculated to penetrate only 90–100 micro m into the slice. Conclusions: Single irradiations of brain tissue, even at high doses of 40 Gy, will result neither in tissue damage visible on a macroscopic level nor necrosis. This is in line with the view that the brain is highly radio-resistant. However, DNA damage can be detected very well in tissue slices using gamma H2AX-immuno staining. Thus, slice cultures are an excellent tool to study radiation-induced damage and repair mechanisms in living tissues.
Y1  - 2009
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7163
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-70998
N1  - © 2009 Müller et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.
IS  - Doc09ptcog142
PB  - German Medical Science GMS Publishing House
CY  - Düsseldorf
ER  -