TY  - JOUR
A1  - Chutharat Samerjai, 
A1  - Kabkaew L. Sukontason, 
A1  - Narin Sontigun, 
A1  - Kom Sukontason, 
A1  - Tunwadee Klong-klaew, 
A1  - Theeraphap Chareonviriyaphap, 
A1  - Kurahashi, Hiromu
A1  - Klimpel, Sven
A1  - Kochmann, Judith
A1  - Atiporn Saeung, 
A1  - Pradya Somboon, 
A1  - Anchalee Wannasan, 
T1  - Mitochondrial DNA-based identification of forensically important flesh flies (Diptera: Sarcophagidae) in Thailand
T2  - Insects
N2  - Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMImin). Thus, the first crucial step is to correctly identify the fly species. Because of the high similarity among species of flesh flies, DNA-based identification is considered more favorable than morphology-based identification. In this study, we demonstrated the effectiveness of combined sequences (2216 to 2218 bp) of cytochrome c oxidase subunit I and II genes (COI and COII) for identification of the following 14 forensically important flesh fly species in Thailand: Boettcherisca nathani Lopes, Fengia ostindicae (Senior-White), Harpagophalla kempi (Senior-White), Liopygia ruficornis (Fabricius), Lioproctia pattoni (Senior-White), Lioproctia saprianovae (Pape & Bänziger), Parasarcophaga albiceps (Meigen), Parasarcophaga brevicornis (Ho), Parasarcophaga dux (Thomson), Parasarcophaga misera (Walker), Sarcorohdendorfia antilope (Böttcher), Sarcorohdendorfia inextricata (Walker), Sarcorohdendorfia seniorwhitei (Ho) and Seniorwhitea princeps (Wiedemann). Nucleotide variations of Thai flesh flies were evenly distributed throughout the COI-COII genes. Mean intra- and interspecific variations ranged from 0.00 to 0.96% and 5.22% to 12.31%, respectively. Using Best Match (BM) and Best Close Match (BCM) criteria, identification success for the combined genes was 100%, while the All Species Barcodes (ASB) criterion showed 76.74% success. Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses yielded similar tree topologies of monophyletic clades between species with very strong support values. The achieved sequences covering 14 forensically important flesh fly species including newly submitted sequences for B. nathani, F. ostindicae and S. seniorwhitei, can serve as a reliable reference database for further forensic entomological research in Thailand and in other areas where those species occur.
KW  - forensic entomology
KW  - molecular identification
KW  - COI
KW  - COII
KW  - flesh flies
KW  - Thailand
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52755
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-527558
SN  - 2075-4450
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 11.2019
IS  - 1, Art. 2
PB  - MDPI
CY  - Basel
ER  -