TY  - JOUR
A1  - Toptan, Tuna
A1  - Hoehl, Sebastian
A1  - Westhaus, Sandra
A1  - Bojkova, Denisa
A1  - Berger, Annemarie
A1  - Rotter, Björn
A1  - Hoffmeier, Klaus
A1  - Cinatl, Jindrich
A1  - Ciesek, Sandra
A1  - Widera, Marek
T1  - Optimized qRT-PCR approach for the detection of intra- abd extra-cellular SARS-CoV-2 RNAs
T2  - International journal of molecular sciences
N2  - The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Meanwhile, increased demand for testing has led to a shortage of reagents and supplies and compromised the performance of diagnostic laboratories in many countries. Both the World Health Organization (WHO) and the Center for Disease Control and Prevention (CDC) recommend multi-step RT-PCR assays using multiple primer and probe pairs, which might complicate the interpretation of the test results, especially for borderline cases. In this study, we describe an alternative RT-PCR approach for the detection of SARS-CoV-2 RNA that can be used for the probe-based detection of clinical isolates in diagnostics as well as in research labs using a low-cost SYBR green method. For the evaluation, we used samples from patients with confirmed SARS-CoV-2 infections and performed RT-PCR assays along with successive dilutions of RNA standards to determine the limit of detection. We identified an M-gene binding primer and probe pair highly suitable for the quantitative detection of SARS-CoV-2 RNA for diagnostic and research purposes.
KW  - SARS-CoV-2
KW  - COVID-19
KW  - qRT-PCR detection
KW  - test protocol
KW  - coronavirus
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54771
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-547710
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
N1  - Preprint erschienen in: bioRxiv beta, 2020, doi:10.1101/2020.04.20.052258
VL  - 21
IS  - 20, Art. 4396
SP  - 1
EP  - 11
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -