TY  - JOUR
A1  - Mosquera, Katherine D.
A1  - Martinez Villegas, Luis E.
A1  - Pidot, Sacha J.
A1  - Sharif, Chinhda
A1  - Klimpel, Sven
A1  - Stinear, Timothy P.
A1  - Moreira, Luciano Augusto dos Santos
A1  - Tobias, Nicholas J.
A1  - Lorenzo, Marcelo
T1  - Multi-omic analysis of symbiotic bacteria associated with Aedes aegypti breeding sites
T2  - Frontiers in microbiology
N2  - Mosquito breeding sites are complex aquatic environments with wide microbial diversity and physicochemical parameters that can change over time during the development of immature insect stages. Changes in biotic and abiotic conditions in water can alter life-history traits of adult mosquitos but this area remains understudied. Here, using microbial genomic and metabolomics analyses, we explored the metabolites associated with Aedes aegypti breeding sites as well as the potential contribution of Klebsiella sp., symbiotic bacteria highly associated with mosquitoes. We sought to address whether breeding sites have a signature metabolic profile and understand the metabolite contribution of the bacteria in the aquatic niches where Ae. aegypti larvae develop. An analysis of 32 mosquito-associated bacterial genomes, including Klebsiella, allowed us to identify gene clusters involved in primary metabolic pathways. From them, we inferred metabolites that could impact larval development (e.g., spermidine), as well as influence the quality assessment of a breeding site by a gravid female (e.g., putrescine), if produced by bacteria in the water. We also detected significant variance in metabolite presence profiles between water samples representing a decoupled oviposition event (oviposition by single females and manually deposited eggs) versus a control where no mosquito interactions occurred (PERMANOVA: p < 0.05; R2 = 24.64% and R2 = 30.07%). Five Klebsiella metabolites were exclusively linked to water samples where oviposition and development occurred. These data suggest metabolomics can be applied to identify compounds potentially used by female Ae. aegypti to evaluate the quality of a breeding site. Elucidating the physiological mechanisms by which the females could integrate these sensory cues while ovipositing constitutes a growing field of interest, which could benefit from a more depurated list of candidate molecules.
KW  - Aedes
KW  - Klebsiella
KW  - breeding sites
KW  - genomics
KW  - metabolomics
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62034
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-620349
SN  - 1664-302X
N1  - This work was funded by the LOEWE-Centre TBG supported by the Hessen State Ministry of Higher Education, Research and the Arts (HMWK), Fundação de Amparo à Pesquisa do Estado de Minas Gerais FAPEMIG – EDITAL 00/2016 – CONFAP – MRC/TEC - APQ-00913-16, CNPq (Project number: 311826/2019-9), INCTEM (Project number: 465678/2014-9), and FIOCRUZ.
VL  - 12
IS  - art. 703711
SP  - 1
EP  - 10
PB  - Frontiers Media
CY  - Lausanne
ER  -