TY  - JOUR
A1  - Eckert, Denise
A1  - Rapp, Felicitas
A1  - Ayele Taddese Tsedeke, 
A1  - Molendowska, Jessica
A1  - Lehn, Robert
A1  - Langhans, Markus
A1  - Fournier, Claudia
A1  - Rödel, Franz
A1  - Hehlgans, Stephanie
T1  - ROS- and radiation source-dependent modulation of leukocyte adhesion to primary microvascular endothelial cells
T2  - Cells
N2  - Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.
KW  - adhesion
KW  - endothelial cells
KW  - inflammation
KW  - leukocytes
KW  - low-dose irradiation
KW  - shear stress
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/81662
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-816628
SN  - 2073-4409
N1  - This research was funded by the German Federal Ministry of Education and Research (BMBF, grant no. 02NUK050A, 02NUK050D; GREWIS alpha). Results are based on experiments in the context of FAIR Phase-0 at GSI, Darmstadt (Germany).
VL  - 11
IS  - 1, art. 72
SP  - 1
EP  - 20
PB  - MDPI
CY  - Basel
ER  -