TY  - JOUR
A1  - Gambaryan, Stepan
A1  - Butt, Elke
A1  - Marcus, Katrin
A1  - Glazova, Margarita
A1  - Palmetshofer, Alois
A1  - Guillon, Gilles
A1  - Smolenski, Albert
T1  - cGMP-dependent protein kinase type II regulates basal level of aldosterone production by zona glomerulosa cells without increasing expression of the steroidogenic acute regulatory protein gene
T2  - Journal of biological chemistry
N2  - The renin-angiotensin-aldosterone system plays a pivotal role in the regulation of salt and water homeostasis. Here, we demonstrate the expression and functional role of cGMP-dependent protein kinases (PKGs) in rat adrenal cortex. Expression of PKG II is restricted to adrenal zona glomerulosa (ZG) cells, whereas PKG I is localized to the adrenal capsule and blood vessels. Activation of the aldosterone system by a low sodium diet up-regulated the expression of PKG II, however, it did not change PKG I expression in adrenal cortex. Both, activation of PKG II in isolated ZG cell and adenoviral gene transfer of wild type PKG II into ZG cells enhanced aldosterone production. In contrast, inhibition of PKG II as well as infection with a PKG II catalytically inactive mutant had an inhibitory effect on aldosterone production. Steroidogenic acute regulatory (StAR) protein that regulates the rate-limiting step in steroidogenesis is a new substrate for PKG II and can be phosphorylated by PKG II in vitro at serine 55/56 and serine 99. Stimulation of aldosterone production by PKG II in contrast to stimulation by PKA did not activate StAR gene expression in ZG cells. The results presented indicate that PKG II activity in ZG cells is important for maintaining basal aldosterone production.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76068
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-760686
SN  - 0021-9258
VL  - 278.2003
IS  - 32
SP  - 29640
EP  - 29648
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -