TY  - JOUR
A1  - Angelini, Roberto
A1  - Vitale, Rita
A1  - Patil, Vinay A.
A1  - Cocco, Tiziana
A1  - Ludwig, Bernd
A1  - Greenberg, Miriam L.
A1  - Corcelli, Angela
T1  - Lipidomics of intact mitochondria by MALDI-TOF/MS
T2  - Journal of lipid research
N2  - A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis. The mitochondrial suspension is directly applied over the target and, after drying, covered by a thin layer of the 9-aminoacridine matrix solution. The lipid profiles acquired with this procedure contain all peaks previously obtained by analyzing the lipid extracts of isolated mitochondria by TLC and/or mass spectrometry. The novel procedure allows the quick, simple, precise, and accurate analysis of membrane lipids, utilizing only a tiny amount of isolated organelle; it has also been tested with intact membranes of the bacterium Paracoccus denitrificans for its evolutionary link to present-day mitochondria. The method is of general validity for the lipid analysis of other cell fractions and isolated organelles.
KW  - cardiolipin
KW  - heart
KW  - Paracoccus denitrificans
KW  - yeast
KW  - 9-aminoacridine
KW  - matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76721
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-767212
SN  - 0022-2275
VL  - 53.2012
IS  - 7
SP  - 1417
EP  - 1425
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -