TY  - JOUR
A1  - White, Helen
A1  - Deprez, Liesbet
A1  - Corbisier, Philippe
A1  - Hall, Victoria
A1  - Lin, Feng
A1  - Mazoua, Stéphane
A1  - Trapmann, Stefanie
A1  - Aggerholm, Anni
A1  - Andrikovics, Hajnalka
A1  - Akiki, Susanna
A1  - Barbany, Gisela
A1  - Boeckx, Nancy
A1  - Bench, Anthony
A1  - Catherwood, Mark
A1  - Cayuela, Jean-Michel
A1  - Chudleigh, Sandra
A1  - Clench, Tim
A1  - Colomer, Dolors
A1  - Daraio, Filomena
A1  - Dulucq, Stephanie
A1  - Farrugia, Joanna
A1  - Fletcher, Linda
A1  - Foroni, Letizia
A1  - Ganderton, Ros
A1  - Gerrard, Gareth
A1  - Gineikienė, Eglė
A1  - Hayette, Sandrine
A1  - El Housni, Hakim
A1  - Izzo, Barbara
A1  - Jansson, Mattias
A1  - Johnels, Petra
A1  - Jurcek, Tomas
A1  - Kairisto, Veli
A1  - Kizilors, Aytug
A1  - Kim, Dong-Wook
A1  - Lange, Thoralf
A1  - Lion, Thomas
A1  - Polakova, Katerina Machova
A1  - Martinelli, Giovanni
A1  - McCarron, Sarah
A1  - Merle, Pauline A.
A1  - Milner, Ben
A1  - Mitterbauer-Hohendanner, Gerlinde
A1  - Nagar, Meital
A1  - Nickless, Guillermina
A1  - Nomdedéu, Josep F.
A1  - Nymoen, Dag A.
A1  - Leibundgut, Elisabeth Oppliger
A1  - Ozbek, Ugur
A1  - Pajič, Tadej
A1  - Pfeifer, Heike
A1  - Preudhomme, Claude
A1  - Raudsepp, Kadri
A1  - Romeo, Giuliana
A1  - Sacha, Tomasz
A1  - Talmaci, Rodica
A1  - Touloumenidou, Tasoula
A1  - Van der Velden, Vincent H. J.
A1  - Waits, Paula
A1  - Wang, Lihui
A1  - Wilkinson, Elizabeth
A1  - Wilson, Gill
A1  - Wren, Dörte
A1  - Zadro, Renata
A1  - Ziermann, Janine
A1  - Zoi, Katerina
A1  - Müller, Martin C.
A1  - Hochhaus, Andreas
A1  - Schimmel, Heinz
A1  - Cross, Nicholas C. P.
A1  - Emons, Hendrik
T1  - A certified plasmid reference material for the standardisation of BCR–ABL1 mRNA quantification by real-time quantitative PCR
T2  - Leukemia, 29.2015, S. 369–376
N2  - Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR–ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 106, 1.08±0.11 × 105, 1.03±0.10 × 104, 1.02±0.09 × 103, 1.04±0.10 × 102 and 10.0±1.5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR–ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR–ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa.eu/jrc/en/reference-materials/catalogue/; CRM code ERM-AD623a-f).
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/37308
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-373087
SN  - 1476-5551
SN  - 0887-6924
N1  - Copyright © 2015 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
VL  - 29
IS  - 2
SP  - 369
EP  - 376
PB  - Nature Publ. Group
CY  - Basingstoke
ER  -