TY  - JOUR
A1  - Lee, Jungho
A1  - Shi, Yi-Ming
A1  - Grün, Peter
A1  - Gube, Matthias
A1  - Feldbrügge, Michael
A1  - Bode, Helge Björn
A1  - Hennicke, Florian
T1  - Identification of feldin, an antifungal polyyne from the beefsteak fungus fistulina hepatica
T2  - Fistulina hepatica. Biomolecules
N2  - Fruiting body-forming members of the Basidiomycota maintain their ecological fitness against various antagonists like ascomycetous mycoparasites. To achieve that, they produce myriads of bioactive compounds, some of which are now being used as agrochemicals or pharmaceutical lead structures. Here, we screened ethyl acetate crude extracts from cultures of thirty-five mushroom species for antifungal bioactivity, for their effect on the ascomycete Saccharomyces cerevisiae and the basidiomycete Ustilago maydis. One extract that inhibited the growth of S. cerevisiae much stronger than that of U. maydis was further analyzed. For bioactive compound identification, we performed bioactivity-guided HPLC/MS fractionation. Fractions showing inhibition against S. cerevisiae but reduced activity against U. maydis were further analyzed. NMR-based structure elucidation from one such fraction revealed the polyyne we named feldin, which displays prominent antifungal bioactivity. Future studies with additional mushroom-derived eukaryotic toxic compounds or antifungals will show whether U. maydis could be used as a suitable host to shortcut an otherwise laborious production of such mushroom compounds, as could recently be shown for heterologous sesquiterpene production in U. maydis.
KW  - agaricomycetes
KW  - antifungals
KW  - biologicals
KW  - polyines
KW  - polyynes
KW  - polyacetylenes
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55341
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-553416
SN  - 2218-273X
VL  - 10
IS  - 1502
PB  - MDPI
CY  - Basel
ER  -