TY  - JOUR
A1  - Hayne, Cassandra K.
A1  - Sekulovski, Samoil
A1  - Hurtig, Jennifer E.
A1  - Stanley, Robin E.
A1  - Trowitzsch, Simon
A1  - Hoof, Ambro van
T1  - New insights into RNA processing by the eukaryotic tRNA splicing endonuclease
T2  - Journal of biological chemistry
N2  - Through its role in intron cleavage, tRNA splicing endonuclease (TSEN) plays a critical function in the maturation of intron-containing pre-tRNAs. The catalytic mechanism and core requirement for this process is conserved between archaea and eukaryotes, but for decades, it has been known that eukaryotic TSENs have evolved additional modes of RNA recognition, which have remained poorly understood. Recent research identified new roles for eukaryotic TSEN, including processing or degradation of additional RNA substrates, and determined the first structures of pre-tRNA-bound human TSEN complexes. These recent discoveries have changed our understanding of how the eukaryotic TSEN targets and recognizes substrates. Here, we review these recent discoveries, their implications, and the new questions raised by these findings.
KW  - tRNA splicing
KW  - RNA
KW  - protein structure
KW  - RNA processing
KW  - RNA degradation
KW  - TSEN
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/77108
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-771086
SN  - 0021-9258
VL  - 299.2023
IS  - 9
SP  - 105138
EP  - 105138
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -