TY  - JOUR
A1  - Trischler, Raphael
A1  - Roth, Jennifer
A1  - Sorbara, Matthew T.
A1  - Schlegel, Xenia
A1  - Müller, Volker
T1  - A functional Wood–Ljungdahl pathway devoid of a formate dehydrogenase in the gut acetogens Blautia wexlerae, Blautia luti and beyond
T2  - Environmental microbiology
N2  - Species of the genus Blautia are typical inhabitants of the human gut and considered as beneficial gut microbes. However, their role in the gut microbiome and their metabolic features are poorly understood. Blautia schinkii was described as an acetogenic bacterium, characterized by a functional Wood–Ljungdahl pathway (WLP) of acetogenesis from H2 + CO2. Here we report that two relatives, Blautia luti and Blautia wexlerae do not grow on H2 + CO2. Inspection of the genome sequence revealed all genes of the WLP except genes encoding a formate dehydrogenase and an electron-bifurcating hydrogenase. Enzyme assays confirmed this prediction. Accordingly, resting cells neither converted H2 + CO2 nor H2 + HCOOH + CO2 to acetate. Carbon monoxide is an intermediate of the WLP and substrate for many acetogens. Blautia luti and B. wexlerae had an active CO dehydrogenase and resting cells performed acetogenesis from HCOOH + CO2 + CO, demonstrating a functional WLP. Bioinformatic analyses revealed that many Blautia strains as well as other gut acetogens lack formate dehydrogenases and hydrogenases. Thus, the use of formate instead of H2 + CO2 as an interspecies hydrogen and electron carrier seems to be more common in the gut microbiome.
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79169
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-791699
SN  - 1462-2920
N1  - This work was supported by the Deutsche Forschungsgemeinschaft (DFG), the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (ACETOGENS, grand agreement no. 741791) and by a Postdoctoral-Faculty Transition award from the Emerald Foundation.
VL  - 24
IS  - 7
SP  - 3111
EP  - 3123
PB  - Blackwell
CY  - Oxford [u.a.]
ER  -