TY  - JOUR
A1  - Berger, Martin
A1  - Maqua, Hendrik
A1  - Lysaja, Katharina
A1  - Mause, Sebastian Frederik
A1  - Hindle, Mathew S.
A1  - Naseem, Khalid
A1  - Dahl, Edgar
A1  - Speer, Thimoteus
A1  - Marx, Nikolaus
A1  - Schütt, Katharina
T1  - Platelets from patients with chronic inflammation have a phenotype of chronic IL-1β release
T2  - Research and practice in thrombosis and haemostasis
N2  - Essentials
• The role of platelet IL-1β release in chronic inflammation is currently unclear.
• Platelets from 65 patients with varying degrees of chronic inflammation were studied.
• Chronic inflammation linked to reduced levels of intracellular IL-1β and IL-1β release.
• Chronic inflammation induces a phenotype that indicates chronic IL-1β release from platelets.
Abstract
Background: Chronic inflammation is a cardiovascular risk factor, and interleukin-1β (IL-1β) is central to the inflammatory host response. Platelets contain the NLRP3 inflammasome and are able to translate IL-1β messenger RNA (mRNA) and secrete mature IL-1β upon activation. However, the role of a chronic inflammatory environment in platelet IL-1β mRNA and protein content remains unclear.
Objectives: The aim of the current study was to investigate intracellular platelet IL-1β and IL-1β mRNA in a chronic inflammatory state.
Methods: Sixty-five patients with stable inflammation (ie, high-sensitivity C-reactive protein within predefined margins in 2 separate measurements) were stratified according to high-sensitivity C-reactive protein levels in low (0.0-0.9 mg/L), medium (1.0-2.9 mg/L), and high (3.0-9.9 mg/L) risk groups. Platelet reactivity as well as platelet IL-1β protein synthesis were studied.
Results: The highest risk group was characterized by a distinct cardiovascular risk profile and approximately 20% higher platelet counts. While platelet reactivity was not different, a reduction in intracellular platelet IL-1β mRNA and IL-1β protein levels was observed in the highest risk group and was linked to decreased platelet size and granularity. This signature suggests a phenotype of chronic IL-1β secretion and could be experimentally phenocopied by stimulation of platelets from healthy volunteers with either TRAP-6 or collagen related peptide (CRP-XL).
Conclusion: Our data suggest a phenotype of chronic IL-1β secretion by platelets in patients with chronic sterile inflammation.
KW  - caspase-1
KW  - high sensitive C-reactive protein
KW  - inflammation
KW  - interleukin-1beta
KW  - platelets
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82784
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-827842
SN  - 2475-0379
VL  - 8.2024
IS  - 1, art. 102261
PB  - Elsevier
CY  - Amsterdam
ER  -